Effect of White Radish on Rat Hepatic Cytochrome P-450 and Theophylline Pharmacokinetics in Rats
Parijat Jain, Drug Delivery and Biotechnology Laboratory, College of Pharmacy and Health Sciences
Jun Shao, Department of Pharmacy and Administrative Sciences, College of Pharmacy and Health Sciences
Abstract
Prescription drugs or medications are a reality in our lives at some time or other. Thus, the opportunity for a food-drug interaction is an every day occurrence. A food-drug interaction can occur when the food affects the ingredients in a medication, preventing the medicine from working the way it should.
White radish (Raphanus sativus) is a popular root vegetable in East Asian countries. One of the long held beliefs among Chinese is that White radish can reduce drug activities. No studies have been performed on the effect of White radish on Cytochrome P450 nor has any white radish - drug interaction been reported, so far, in the literature. Thus our study design was aimed to determine the effect of White radish on metabolizing activity of rat hepatic microsomal Cytochrome P450 and on Theophylline pharmacokinetics in rats.
The effect of White Radish (5.0 g/kg body weight for 5 days by oral gavage) on metabolizing activity of rat hepatic microsomal Cytochrome P450 was determined using liver weight, hepatic microsomal heme quantification, hepatic Cytochrome P450 content and hepatic Cytochrome P450 3A4 activity. No significant differences were observed between treated and control groups relating to the above parameters measured. These results show that White Radish at a dose of 5.0 mg/kg body weight did not affect the hepatic microsomal drug metabolizing enzyme Cytochrome P450 activity.
The effect of White radish (5.0 g/kg body weight for 5 days by oral gavage) on Theophylline pharmacokinetics in rats was determined using various pharmacokinetic parameters viz. distribution rate constant (á), elimination rate constant (â), area under the curve (AUC), clearance (Cl), elimination half-life (t1/2 â) and total volume of distribution (Vdt). A mean plasma concentration-time profile of Theophylline in rats was obtained. The above mentioned parameters were not found to be significantly different. Hence, it can be concluded that White radish at a dose of 5.0 g/kg body weight did not affect the pharmacokinetics of theophylline in rats after bolus administration through the tail vein at a dose of 7.5 mg/kg body weight.